Downregulation of microRNA1835p inhibits the proliferation and invasion of colorectal cancer cells by inactivating the reticulocalbin2/Wnt/βcatenin signaling pathway

microRNAs (miRNAs) are frequently aberrantly expressed in colorectal cancer (CRC) and are considered to serve a critical role in the onset and development of CRC by binding to its target transcription factor. The aim of the present study was to examine the role of miRNA (miR)1835p in the proliferation, invasion and migration of CRC cells, in addition to its underlying mechanism. Reverse transcriptionquantitative polymerase chain reaction analysis was used to detect the expression level of miR1835p. MTT and Transwell assays were performed to examine proliferation and invasion in SW620 cells. Western blot analysis was performed to determine the protein expression of reticulocalbin2 (RCN2), matrix metalloproteinase2, βcatenin, cyclin D1 and cMyc. miR1835p expression was significantly upregulated in the CRC tissues compared with adjacent normal tissues. In addition, the inhibition of miR1835p suppressed proliferation, invasion and migration in SW620 cells. miR1835p downregulation or overexpression regulated the CRC cell cycle, invasion and migration by modulating RCN2 expression. Furthermore, the Wnt/βcatenin pathway was observed to be involved in the inhibitory effect of miR1835p downregulation in CRC cell proliferation, invasion and migration. These results provided evidence that the downregulation of miR1835p inhibits CRC proliferation and invasion by regulating the RCN2/Wnt/βcatenin pathway. miR1835p and RCN2 may serve an important role in the molecular etiology of CRC and have potential applications in CRC treatment.