Gypenosides attenuate cholesterolinduced DNA damage by inhibiting the production of reactive oxygen species in human umbilical vein endothelial cells
Previous studies have demonstrated that DNA damage induces atherosclerosis and that oxidative stress has an important role in DNA damage. Gypenosides (Gps), the main ingredient of Gynostemma Pentaphylla (Thunb.) Makino, have been recognized as specific antioxidants and have previously
been reported to inhibit highfat dietinduced atherosclerosis in rats. However, whether or not Gps attenuate DNA damage through their antioxidant effects remains to be elucidated. The current study was performed to clarify whether or not Gps can inhibit cholesterolinduced DNA damage through
antioxidation. The present study provided new insights into the pharmacological effects of Gps on atherosclerosis. HUVECs were treated with Gps at various concentrations (1, 10 and 100 µg/ml) for 1 h. The protective effects of Gps on cholesterolinduced DNA damage were determined
using immunofluorescence, western blotting, reversetranscription quantitative polymerase chain reaction and flow cytometry. Pretreatment with Gps (1, 10 and 100 µg/ml) effectively attenuated cholesterolinduced DNA damage in HUVECs by inhibiting phosphorylation of H2AX, a member
of the histone family. Furthermore, Gps (100 µg/ml) pretreatment inhibited cholesterolinduced transcription and activity of nicotinamide adenine dinucleotide phosphateoxidase 4 and reduced intracellular ROS levels. In conclusion, Gps attenuated cholesterolinduced DNA damage
by inhibiting ROS production in HUVECs, suggesting that the inhibitory effect of Gps on atherogenesis is correlated with the alleviation of DNA damage.
Document Type: Research Article
Affiliations: 1: Department of Biochemistry, Zunyi Medical College, Zunyi, Guizhou 563000, P.R. China 2: Department of Pharmacology, Zunyi Medical College, Zunyi, Guizhou 563000, P.R. China
Publication date: 01 January 2015
- Molecular Medicine Reports is a monthly, peer-reviewed journal available in print and online, that includes studies devoted to molecular medicine, underscoring aspects including pharmacology, pathology, genetics, neurosciences, infectious diseases, molecular cardiology and molecular surgery. In vitro and in vivo studies of experimental model systems pertaining to the mechanisms of a variety of diseases offer researchers the necessary tools and knowledge with which to aid the diagnosis and treatment of human diseases.
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