Production, Purification and Characterization of Adeno-Associated Vectors
Keywords: A549; AAV serotype 2; AAV7; AAV8; AAV9; AVB Sepharose High performance medium; CD8 T cell; Cesium chloride (CsCl) isopycnic gradient centrifugation; Column chromatography; E1B genes; E2A; E4; ELISA; GMP; Gene therapy; HEK 293 cells; HEK293 cell lines; HeLa; Hela cells; ITR; Nhydroxysuccinimide (NHS)-activated Sepharose High Performance; Parvoviridae; RPE65 deficiency; Rep 68; Rep 78; Transduction Titer; VP1; VP2; VP3; Vector Titration; adenoviruses (Ad); anion-exchange chromatography; baculovirus vectors; benzonase; cap gene; chromosome 19; empty capsids; gradient vs. column purification; helper viruses; herpes simplex virus (HSV); human factor IX transgene; p19; p19 promoter; p5; p5 promoter; polyacrylamide gel electrophoresis (PAGE); polyethylene glycol (PEG); vaccinia virus, and cytomegalovirus (CMV); virus-associated (VA) RNA
Document Type: Research Article
Publication date: 01 December 2010
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