Tail fan necrosis (TFN) is an increasingly common condition found in commercially-fished and aquacultured spiny lobsters. Chitin, lipid, and protein are the major components of the lobster cuticle, and we test whether bacteria proteolytic, chitinolytic, and lipolytic activities are
associated with the presence of TFN in spiny lobsters. Bacteria were cultured from the surface of the tail fans and from the hemolymph of wild Australasian red spiny lobsters, Jasus edwardsii (Hutton, 1875), with TFN and from those without any signs of TFN. The proteolytic, chitinolytic,
and lipolytic activities of 1678 bacteria isolates were characterized using standard plating methods. Chitinolytic bacteria strains were more common on the tail fans and in the hemolymph of lobsters with TFN compared with those without TFN, and the overall chitinolytic activity of bacteria
present in lobsters with TFN was higher than in lobsters without TFN. The bacteria isolates with a combination of proteolytic, chitinolytic and lipolytic activities were more prevalent on the tail fans and in the hemolymph of lobsters with TFN. Molecular methods identified 14 bacteria species
from 35 isolates possessing this broad enzymatic profile associated with TFN with over 50% of the isolates belonging to four species in the Splendidus clade of Vibrio, which are known aquatic pathogens. Collectively these results suggest a suite of bacteria exhibiting proteolytic, chitinolytic
and lipolytic activities were associated with TFN in lobsters. These bacteria may be competitively advantaged in the opportunistic infection of sites of physical damage to the protective cuticle in spiny lobsters.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
School of Biological Sciences, University of Auckland, New Zealand, Institute of Marine Science, University of Auckland, New Zealand; or, [email protected], Email: [email protected]
School of Biological Sciences, University of Auckland, New Zealand, Institute of Marine Science, University of Auckland, New Zealand
School of Biological Sciences, University of Auckland, New Zealand
Publication date: 01 July 2018
This article was made available online on 22 May 2018 as a Fast Track article with title: "Characteristics of culturable bacteria associated with tail fan necrosis in the spiny lobster, Jasus edwardsii".
More about this publication?
The Bulletin of Marine Science is dedicated to the dissemination of high quality research from the world's oceans. All aspects of marine science are treated by the Bulletin of Marine Science, including papers in marine biology, biological oceanography, fisheries, marine affairs, applied marine physics, marine geology and geophysics, marine and atmospheric chemistry, and meteorology and physical oceanography.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- Ingenta Connect is not responsible for the content or availability of external websites