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Open Access The Metal Catalyst Influences the Kinetics and Mechanisms of MS2 Inactivation in Fenton-like Systems

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This article is Open Access under the terms of the Creative Commons CC BY-NC licence.

Human enteric viruses are frequent microbial contaminants of surface water and groundwater. Waterborne viruses can be effectively inactivated by oxidants, such as those generated in Fenton-like systems. However, the mechanisms by which this inactivation occurs are not understood. Here we investigated how two Fenton-like systems, Cu/H2O2 and Fe/H2O2/light, affect the infectivity and structural integrity of MS2 coliphage, a frequently used surrogate for human enteric viruses. The extent of MS2 genome and capsid protein degradation was evaluated by quantitative PCR and protein mass spectrometry, and was related to the observed level of inactivation. Even though inactivation in both systems occurred via the same oxidant, hydroxyl radical, the contributions of genome and capsid protein degradation to inactivation differed. Inactivation in the Cu/H2O2 system was rapid and involved both genome and protein damage. In contrast, inactivation in Fe/H2O2/light proceeded at a slower rate and encompassed solely genome damage. Our findings demonstrate that not only the oxidant, but also its source, the metal catalyst, determines the inactivation kinetics and mechanism in Fenton-like systems. This work provides the first evidence of the impact of the metal catalyst on virus inactivation in Fenton-like systems.
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Keywords: DISINFECTION; FENTON; INACTIVATION MECHANISMS; PROTEIN MASS SPECTROMETRY; WATERBORNE VIRUS

Document Type: Research Article

Affiliations: 1: Laboratory of Environmental Chemistry, School of Architecture, Civil and Environmental Engineering (ENAC), École Polytechnique Fédérale de Lausanne (EPFL), Station 2, CH-1015 Lausanne;, Email: [email protected] 2: Laboratory of Environmental Chemistry, School of Architecture, Civil and Environmental Engineering (ENAC), École Polytechnique Fédérale de Lausanne (EPFL), Station 2, CH-1015 Lausanne

Publication date: March 1, 2020

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  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.

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