Skip to main content
padlock icon - secure page this page is secure

Open Access High-spin Metal Centres in Dipolar EPR Spectroscopy

Download Article:
(PDF 339.4 kb)
The substitution of Mg2+ by Mn2+ in the bacterial DnaB helicase from Helicobacter pylori, an ATP:Mg2+-fuelled protein engine, allows electron paramagnetic resonance (EPR) spectroscopy to be performed on this system. EPR experiments make it possible to monitor nucleotide binding and to estimate the fraction of bound Mn2+ through relaxation measurements. Furthermore, by measuring spin–spin distances we probe the geometry within such multimeric assemblies using ultra-wideband double electron-electron resonance (DEER) and relaxation induced dipolar modulation enhancement (RIDME). The extraction of distance distributions from RIDME experiments on high-spin paramagnetic centres is influenced by the presence of dipolar frequency overtones. We show herein that we can correct for these overtones by using a modified kernel function in Tikhonov regularization analysis routines, and that the overtone coefficients for Mn2+ in the DnaB helicase are practically the same as in the previously studied Mn2+–Mn2+ model compounds.

49 References.

No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Affiliations: 1: ETH Zurich, Laboratorium für Physikalische Chemie, Vladimir-Prelog-Weg 2, CH-8093 Zurich, Switzerland 2: Molecular Microbiology and Structural Biochemistry, Labex Ecofect UMR 5086 CNRS/Université de Lyon, Lyon, France 3: ETH Zurich, Laboratorium für Physikalische Chemie, Vladimir-Prelog-Weg 2, CH-8093 Zurich, Switzerland. [email protected]

Publication date: April 1, 2018

More about this publication?
  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Information for Advertisers
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more