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Open Access Directed Evolution of Artificial Metalloenzymes: Genetic Optimization of the Catalytic Activity

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This article is Open Access under the terms of the Creative Commons CC BY-NC-ND licence.

Artificial metalloenzymes (ArMs) based on the incorporation of a biotinylated metal cofactor within a streptavidin (Sav) combine attractive features of both enzymatic and homogeneous catalysis. To speed up their optimization, we present a directed evolution of an artificial transfer hydrogenase (ATHase) based on a stream-lined and optimized protocol for the design, overexpression and screening of Sav isoforms. Ten positions have been subjected to mutagenesis to yield two variants with improved catalytic activity and selectivity for the reduction of cyclic imines, along with greater stability in a biphasic medium.
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Keywords: ARTIFICIAL METALLOENZYMES; CYCLIC IMINES; DIRECTED EVOLUTION; ENZYME CATALYSIS; TRANSFER HYDRO-GENATION

Document Type: Research Article

Affiliations: Department of Chemistry, University of Basel, Mattenstrasse 24a, BPR 1096, CH-4002 Basel, Switzerland. [email protected]

Publication date: 01 April 2018

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  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.

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