Enhanced Enzyme Performance by DNA Shuffling
DNA shuffling includes in vivo and in vitro methods for recombination of nucleic acid sequences. In one format, DNA shuffling involves methods for in vitro homologous recombination of pools of related genes. For example, fragmentation of nucleic acid sequences that encode genes, can be followed by reassembly using the polymerase chain reaction (PCR). As the complete gene sequence can be shuffled, a wider sequence space can be accessed in searching for the desired, improved variant. Repeated cycles of recombination, optionally together with error-prone PCR to introduce point mutations, allow efficient molecular evolution of complex sequences in vitro, to provide biological molecules with improved properties.
Document Type: Research Article
Publication date: December 1, 1999
International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions
CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.
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