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Open Access Enzymatic Synthesis of β-D-Glucuronides in an Enzyme Membrane Reactor

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The production of two O-glucuronides in an enzyme membrane reactor on a 100 to 200 mg scale was examined. The aglycons were conjugated with the co-substrate β-D-uridine diphosphoglucuronic acid (UDPGA) in the presence of a guinea-pig liver preparation. The continuous synthesis, which was run in an enzyme membrane reactor, was followed depending on the substrate up to 118 hours or 24 hours, respectively. The reaction was monitored by TLC or HPLC. The purification of the two glucuronides was carried out by ion-exchange chromatography and by reversed-phase HPLC.
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Document Type: Research Article

Publication date: December 1, 1999

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  • International Journal for Chemistry and Official Membership Journal of the Swiss Chemical Society (SCS) and its Divisions

    CHIMIA, a scientific journal for chemistry in the broadest sense, is published 10 times a year and covers the interests of a wide and diverse readership. Contributions from all fields of chemistry and related areas are considered for publication in the form of Review Articles and Notes. A characteristic feature of CHIMIA are the thematic issues, each devoted to an area of great current significance.

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