Skip to main content

Factors associated with false-negative for T-SPOT.TB in patients with TB disease

Buy Article:

$34.78 + tax (Refund Policy)

BACKGROUND:: T-SPOT.TB is an adjunctive diagnostic tool that is used for detecting TB disease. However, in some patients with TB disease, a false-negative result can occur. This study aims to determine the factors that are associated with false-negative T-SPOT.TB results in TB disease.

METHODS:: We retrospectively enrolled 210 patients who were diagnosed with TB disease and underwent T-SPOT.TB testing between July 2014 and July 2020 at Ramathibodi Hospital Bangkok, Thailand. Clinical characteristics, laboratory findings and radiographic findings were compared between the false-negative and true-positive T-SPOT.TB groups.

RESULTS:: Among 210 patients with TB disease, 144 patients had pulmonary TB, 44 patients had extrapulmonary TB and 22 patients had concomitant pulmonary and extrapulmonary TB. There were 100 male and 110 female patients, with a mean age of 54.1 ± 17.5 years. Ninety patients (42.9%) had false-negative T-SPOT.TB results. Multivariate analysis showed that increased age was significantly associated with false-negative T-SPOT.TB (OR 1.025, 95% CI 1.008–1.043, P = 0.006). Those aged ≥55 years had 1.8 times higher false-negative T-SPOT.TB rate than the younger group (age ≥55 years: 64.4% vs. age <55 years: 35.6%; P = 0.001).

CONCLUSION:: Advanced age, particularly age ≥55 years, was a significant factor that related with false-negative T-SPOT.TB results in TB disease.

Keywords: T-SPOT.TB; active tuberculosis; false-negative; interferon gamma release assays; tuberculosis disease

Document Type: Research Article

Affiliations: Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand

Publication date: February 1, 2023

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease (IJTLD) is for clinical research and epidemiological studies on lung health, including articles on TB, TB-HIV and respiratory diseases such as COVID-19, asthma, COPD, child lung health and the hazards of tobacco and air pollution. Individuals and institutes can subscribe to the IJTLD online or in print – simply email us at [email protected] for details.

    The IJTLD is dedicated to understanding lung disease and to the dissemination of knowledge leading to better lung health. To allow us to share scientific research as rapidly as possible, the IJTLD is fast-tracking the publication of certain articles as preprints prior to their publication. Read fast-track articles.

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • International Journal of Tuberculosis and Lung Disease
  • Public Health Action
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content