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Home / The International Journal of Tuberculosis and Lung Disease, Volume 15, Number 12

Molecular characterisation of isoniazid- and rifampicin-resistant Mycobacterium tuberculosis in Central Tunisia

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The aim of our study was to genotypically characterise isoniazid (INH) and rifampicin (RMP) resistant Mycobacterium tuberculosis isolates in Sousse, Central Tunisia, using DNA sequencing and multispacer sequence typing (MST). The results show that 27/28 (96.4%) and 1/28 (3.6%) INH-resistant isolates yielded respectively the kat G S315T and the inh A – 15C → T mutations. Two-thirds of RMP-resistant isolates yielded the rpo B D516V mutation and one sixth yielded either H526D or S531L mutations. Genotyping analysis revealed the multiclonal spread of drug-resistant isolates in Central Tunisia. Data presented here complete the previously published map of resistant M. tuberculosis isolates and highlight their regional disparity in Tunisia.

Keywords: DNA SEQUENCING; MULTIDRUG RESISTANCE; TUBERCULOSIS

Document Type: Research Article

Affiliations: 1: Laboratoire de Microbiologie et d'Immunologie, UR02/SP13, CHU Farhat Hached Sousse, Tunisie; Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Unité Mixte de Recherche 6236, Institut de Recherche pour le Développement 198, Institut Fédératif de Recherche 48, Centre National de la Recherche Scientifique, Faculté de Médecine, Université de la Méditerranée, Marseille, France 2: Laboratoire de Microbiologie et d'Immunologie, UR02/SP13, CHU Farhat Hached Sousse, Tunisie 3: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Unité Mixte de Recherche 6236, Institut de Recherche pour le Développement 198, Institut Fédératif de Recherche 48, Centre National de la Recherche Scientifique, Faculté de Médecine, Université de la Méditerranée, Marseille, France 4: Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Unité Mixte de Recherche 6236, Institut de Recherche pour le Développement 198, Institut Fédératif de Recherche 48, Centre National de la Recherche Scientifique, Faculté de Médecine, Université de la Méditerranée, Marseille, France

Publication date: 01 December 2011

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