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Free Content Evaluation of a rapid assay for identifi cation of Mycobacterium tuberculosis grown in solid and liquid media

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An MPT64 antigen detection assay, the SD Bioline TB Ag MPT64 Rapid kit, was evaluated for the identification of Mycobacterium tuberculosis grown in both solid and liquid media. The rapid test showed positive results in 132 of 133 (99.2%) M. tuberculosis cultures grown on Ogawa medium and 136/143 (95.1%) cultures grown in liquid culture medium, resulting in an overall sensitivity of 97.1%. All 18 non-tuberculous mycobacteria were found to be negative by the rapid test, indicating a specificity of 100%. The rapid test seems useful for the initial confirmation of M. tuberculosis in acidfast bacilli positive cultures.
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Keywords: IDENTIFICATION; MPT64 ANTIGEN; MYCOBACTERIUM TUBERCULOSIS

Document Type: Research Article

Affiliations: 1: Infectious Disease Section, Department of Medicine, University of the Philippines–Philippine General Hospital, Manila, Philippines 2: Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju, South Korea 3: Department of Microbiology and Institute of Immunology and Immunological Disease, Yonsei University College Medicine, Seoul, South Korea

Publication date: November 1, 2011

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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