SETTING: The mammalian cell entry (mce) proteins Mce3A, Mce3D and Mce3E, encoded by the mce3 operon of Mycobacterium tuberculosis, have recently been shown to be expressed during natural infection in humans. OBJECTIVE: To determine the potential of Mce3A, Mce3D and Mce3E proteins in the serodiagnosis of tuberculosis (TB). DESIGN: The quantitative detection of anti-Mce3A, -Mce3D and -Mce3E antibodies in serum samples from active TB patients (n = 58), healthy contacts of TB patients (n = 24) and bacilli Calmette-Guérin (BCG) vaccinated healthy subjects (n = 24) was performed using enzyme-linked immunosorbent assay (ELISA). RESULTS: Antibodies in serum from 98%, 86% and 90% of active TB patients and from 92%, 75% and 96% of healthy contacts of TB patients reacted with Mce3A, Mce3D and Mce3E proteins, respectively. However, none of the serum from BCG-vaccinated healthy subjects reacted with Mce3A and Mce3E proteins, and only 8% of serum samples reacted with Mce3D protein. Overall, serum from 98% active TB patients, 96% healthy contacts and 0% BCG-vaccinated healthy subjects were positive for anti-Mce3A and/or -Mce3E antibodies. CONCLUSIONS: Our results suggest that Mce3A and Mce3E proteins may be useful for the serodiagnosis of TB infection.
No Reference information available - sign in for access.
No Supplementary Data.
No Article Media
active TB disease;
Document Type: Regular Paper
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait
June 1, 2007
More about this publication?
The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on lung health world-wide.
To share scientific research of immediate concern as rapidly as possible, The Union is fast-tracking the publication of certain articles from the IJTLD and publishing them on The Union website, prior to their publication in the Journal. Read fast-track articles.
Certain IJTLD articles are also selected for translation into French, Spanish, Chinese or Russian. These are available on the Union website.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- International Journal of Tuberculosis and Lung Disease
- Public Health Action
- Ingenta Connect is not responsible for the content or availability of external websites