Evaluation of a new automated, rapid, colorimetric culture system using solid medium for laboratory diagnosis of tuberculosis and determination of anti-tuberculosis drug susceptibility
OBJECTIVE: To evaluate the performance of the Dio-TK Culture System (CS), a new, rapid, automated colorimetric culture system.
DESIGN: Dio-TK CS results were compared with routinely used Löwenstein Jensen (LJ) medium and Bactec 460 TB.
RESULTS: In this study, 449 specimens, mostly sputum samples obtained from 348 patients, were evaluated. Mycobacteria were isolated from 31 (6.9%), 23 (5.1%), 18 (4.0%) and 21 (4.7%) of the specimens using Bactec 12B, LJ, Dio-TK Medium and Dio-TK SLC (selective), respectively. The mean time to detection of growth of 13 isolates by Bactec 12B, Dio-TK Medium, Dio-TK SLC and LJ medium was respectively 8.9, 15.1, 17.0 and 26.1 days.
CONCLUSION: Dio-TK may be a practical and rapid culture system for daily use. However, the manufacturer should improve the system to minimise the effects of manipulation errors. Comparative studies with a larger number of isolates are needed to standardise drug concentrations used in anti-tuberculosis drug susceptibility testing.
Document Type: Regular Paper
Affiliations: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey
Publication date: 01 June 2004
The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.
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