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Open Access Varietal identification of Thai hot chilli using seed protein analysis by ultrathin layer isoelectric focusing technique

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This article is Open Access under the terms of the Creative Commons CC BY licence.

This research aimed to determine the protein extraction solvent and pH of gel acrylamide that are suitable for conducting varietal verification of Thai hot chilli by ultrathin layer isoelectric focusing. Four extraction solvents, EX 3 (urea + ethylene glycol), phosphate buffer, water and HEPES, and two gel pH ranges, 3-10 and 5-8/2-11, forming eight treatment combinations were compared. Ten chilli cultivars, Capsicum annuum 'Hua reur yon', 'Mun', 'Gaw', 'Pama' and 'Yod son lek' and C. frutescens 'Mong', 'She deuy kai', 'Pop puang', 'Som ka rieang' and 'Kee noo suan', were tested. Protein extraction with EX 3 in combination with gel pH 3-10 gave the best varietal verification of chilli varieties in this study. In pairwise comparisons of the 10 chilli varieties, this treatment combination was able to verify all the 45 pairs with 100% efficacy.
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Keywords: CAPSICUM ANNUUM; CAPSICUM FRUTESCENS; EXTRACTION SOLVENT; GEL pH; THAI HOT-CHILLI

Document Type: Research Article

Publication date: July 1, 2019

This article was made available online on August 15, 2019 as a Fast Track article with title: "Varietal identification of Thai hot chilli using seed protein analysis by ultrathin layer isoelectric focusing technique".

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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