Plasma membrane permeability is an important character of dry seeds It indicates the degree of membrane integrity and hence seed quality. The molecular mechanisms that affect membrane permeability and repair during imbibition are not well understood. Seeds of wheat varieties 'Yuan 53646'
and 'Luo Han 8', identified as differing in membrane permeability, were used for transcriptome sequencing of the embryos before (dry seeds) and after six hours imbibition. Comparative transcriptome analysis was performed. 1,354 significant differentially expressed genes (DEGs) in dry seeds
and 728 significant DEGs in 6-hour imbibed seeds were identified. KEGG enrichment analysis revealed that "Protein processing in endoplasmic reticulum" (ko04141) was significantly enriched in dry 'Yuan 53646' seeds, suggesting that it may participate in protecting or repairing membranes. 15
DEGs, involved in water stress response, lipid body transport and membrane-bound vesicular transport, calcium ion binding and wound-induced proteins were identified as possible genes related to membrane integrity. Quantitative real-time PCR successfully validated the transcriptome analysis.
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DIFFERENTIALLY EXPRESSED GENES;
Document Type: Research Article
April 1, 2019
This article was made available online on March 23, 2019 as a Fast Track article with title: "Differences in embryo membrane permeability and gene expression between wheat varieties ‘Luo Han 8’ and ‘Yuan 53646’".
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