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Open Access The Potential of ROS Inhibitors and Hydrated Storage in Improving the Storability of Recalcitrant Madhuca Latifolia Seeds

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The storage of recalcitrant seeds represents a challenge globally, threatening the germplasm conservation of many economically important tropical and sub-tropical species. This study investigated whether inhibitors of reactive oxygen species (ROS), diphenyleneiodonium (DPI) and dimethyl thiourea (DMTU), and hydrated storage can enhance the storability of Madhuca latifolia seeds. Treatment of M. latifolia seeds with varied concentrations of DPI and DMTU solutions revealed 150 μM and 3000 μM to be ideal concentrations, respectively. Soaking seeds in aqueous solutions of DPI and DMTU, and MilliQ water (MW; control) increased seed water content (WC), so seeds were initially dried back to their original WC, before hydrated storage at 25 and 16°C. A decline in germination (37%) and WC (42%) was observed after 69 days of hydrated storage at 25°C, and complete loss of viability after 120 days. A 50% decline in germination was recorded after just 19 days of hydrated storage at 16°C which is possibly indicative of sensitivity to low temperatures. Even though WC did not decline significantly at 16°C over the entire storage period, all viability was lost after 90 days. The ROS inhibitors did not extend storage lifespan relative to the control but reduced the rate of in situ germination during storage, without compromising viability. Hydrated storage at 25°C, coupled with pre-treatment with ROS inhibitors, is recommended for the short- to medium-term storage of M. latifolia seeds.

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Keywords: DESICCATION; GERMINATION; MADHUCA LATIFOLIA; SEED LONGEVITY; VIABILITY; VIGOUR; WATER CONTENT

Document Type: Research Article

Publication date: April 1, 2019

This article was made available online on March 14, 2019 as a Fast Track article with title: "The potential of ROS inhibitors and hydrated storage in improving the storability of recalcitrant Madhuca latifolia seeds".

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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