Progress curves of the germination (radicle emergence, RE) of 12 seed lots of oilseed rape were determined manually in two laboratories (Alexander Harley Seeds Ltd. [Harleys] and GEVES) over 14 days, as was normal germination (%). RE was also determined automatically using image analysis
(GEVES) over five days. MGT from manual counts was highly predictive of normal seedlings, both for all lots and lots having > 65% normal seedlings (R
2 = 0.95, Harleys; 0.71 GEVES). Single RE counts were also predictive of MGT (R
2 > 0.90), and normal
2 = 0.92 at Harleys (2-day RE) and 0.77 at GEVES (3-day RE). Five lots with > 90% normal seedlings were identified by RE counts of > 75% in both laboratories. Automated early RE counts gave similar predictive relationships. The electrical conductivity (EC)
of seed soak water after 24 hours predicted normal seedlings (%): R
2 = 0.87, GEVES; 0.79, Harleys. All lots that produced > 90% normal seedlings had an EC below 120 μS cm-1 g-1. These predictive relationships are explained in terms of an ageing
/ repair hypothesis. Single counts of RE after two days and 24-hour EC are proposed as quick and inexpensive predictors of normal seedlings in a germination test.
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ELECTRICAL CONDUCTIVITY TEST;
PREDICTION OF NORMAL SEEDLINGS;
RADICLE EMERGENCE TEST
Document Type: Research Article
April 1, 2018
This article was made available online on December 14, 2017 as a Fast Track article with title: "Potential for early counts of radicle emergence and leakage of electrolytes as quick tests to predict the percentage of normal seedlings".
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