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Open Access Potential for early counts of radicle emergence and leakage of electrolytes as quick tests to predict the percentage of normal seedlings

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Progress curves of the germination (radicle emergence, RE) of 12 seed lots of oilseed rape were determined manually in two laboratories (Alexander Harley Seeds Ltd. [Harleys] and GEVES) over 14 days, as was normal germination (%). RE was also determined automatically using image analysis (GEVES) over five days. MGT from manual counts was highly predictive of normal seedlings, both for all lots and lots having > 65% normal seedlings (R 2 = 0.95, Harleys; 0.71 GEVES). Single RE counts were also predictive of MGT (R 2 > 0.90), and normal seedlings: R 2 = 0.92 at Harleys (2-day RE) and 0.77 at GEVES (3-day RE). Five lots with > 90% normal seedlings were identified by RE counts of > 75% in both laboratories. Automated early RE counts gave similar predictive relationships. The electrical conductivity (EC) of seed soak water after 24 hours predicted normal seedlings (%): R 2 = 0.87, GEVES; 0.79, Harleys. All lots that produced > 90% normal seedlings had an EC below 120 μS cm-1 g-1. These predictive relationships are explained in terms of an ageing / repair hypothesis. Single counts of RE after two days and 24-hour EC are proposed as quick and inexpensive predictors of normal seedlings in a germination test.
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Document Type: Research Article

Publication date: April 1, 2018

This article was made available online on December 14, 2017 as a Fast Track article with title: "Potential for early counts of radicle emergence and leakage of electrolytes as quick tests to predict the percentage of normal seedlings".

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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