Siberian wildrye (Elymus sibiricus) is an important perennial grass used for reclamation of degraded rangeland and hay production. However, the practical value of the seeds decrease under adverse storage conditions. This study was conducted on seeds subjected to two different
ageing conditions - controlled deterioration (CD) at 45°C for 48 hours (seeds with 4, 10 and 16% moisture content, MC) and accelerated ageing (AA) at 45°C and 100% relative humidity for 0, 24, 48 and 72 hours (seeds with 10.8% MC) - to investigate effects of glutathione (GSH) on seed
resistance against ageing by measuring germination, antioxidant enzyme activities, lipid peroxidation and embryonic cellular ultrastructure. Glutathione alleviated the loss of seed germinability both under CD and AA, and correspondingly, mitigated the increase in electrical conductivity (EC)
and malondialdehyde (MDA). Glutathione also had a protective effect on ultrastructural integrity of embryonic cells, especially when seeds were held at 10% MC in CD or aged for 48 hours in AA. Furthermore, activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione
reductase (GR) in seeds under CD were also increased by GSH. Glutathione pre-treatment made some contributions for improving germination and resistance against ageing in Elymus sibiricus seeds subjected to CD and AA conditions.
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Document Type: Research Article
December 1, 2017
This article was made available online on October 4, 2017 as a Fast Track article with title: "Exogenous glutathione pre-treatment improves germination and resistance of Elymus sibiricus seeds subjected to different ageing conditions".
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