Methods for rapid determination of pecan (Carya illinoensis) seed viability
Different preconditioning treatments, tetrazolium concentrations, staining temperature and staining time were evaluated for viability testing Carya illinoensis seeds. The best preconditioning method was taking a quarter-nut with the completely exposed radicle. MRI proved that the reason why radicle could not be stained was because water and TZ was unable to permeate the radicle and surrounding region of the nut when the radicle is not completely exposed. Pecan seeds stain well over a wide range of conditions. Staining with 0.5% TZ solution at 35°C required just four hours which is faster than other treatments. Contrasting with the germination of five seed lots, five staining patterns were recognised; two staining patterns indicated viable seeds and the other three patterns indicated non-viable seeds.
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Document Type: Research Article
Publication date: July 1, 2017
This article was made available online on June 16, 2017 as a Fast Track article with title: "Methods for rapid determination of pecan (Carya illinoensis) seed viability".
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