This experiment was designed to measure the changes in ultrastructure, antioxidant enzymatic activities and lipid peroxidation in ultra-dried (4% moisture content) and artificially aged (48 days at 45°C) oat (Avena sativa L.) seeds. The seeds were also primed with
polyethylene glycol (PEG) solutions (0, -0.3, -0.6, -0.9 and -1.2 MPa) for 12 hours at 20°C. The results showed that seed vigour, antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and glutathione reductase (GR), and the
ultrastructure of the embryo cells were improved at lower water potential, whilst the malondialdehyde (MDA) contents decreased significantly (P < 0.05). This indicated that the effect of PEG-priming on seed vigour was positively related to antioxidant enzymes and ultrastructure of
embryo cells, but was negatively related to MDA content. In terms of ultrastructure, the mitochondria were the most sensitive organelles to PEG-priming. PEGpriming at 0, -0.3 and -0.6 MPa aggravated the damage of seed ageing, but priming at -0.9 and -1.2 MPa was beneficial. It is therefore
recommended that the water potential used for priming with PEG should be less than -0.9 MPa for ultra-dried aged seeds.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
December 1, 2016
This article was made available online on October 26, 2016 as a Fast Track article with title: "Antioxidant and ultrastructural responses to priming with PEG in aged, ultra-dry oat seed".
More about this publication?
Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Membership Information
- Ingenta Connect is not responsible for the content or availability of external websites