Skip to main content
padlock icon - secure page this page is secure

Assessment of germination and endophyte viability in perennial ryegrass and tall fescue seeds following accelerated ageing treatment

Buy Article:

$35.00 + tax (Refund Policy)

Temperate pasture grasses form symbiotic associations with asexual fungal endophytes of the genus Epichloë. Viabilities of both seed and endophyte were assessed in cultivars of perennial ryegrass and tall fescue following different accelerated ageing (AA) treatments based on variations of relative humidity (RH) (80-100%) and duration at elevated temperature (42°C), to identify particularly stable and compatible host-endophyte associations. The cultivars were inoculated with three endophytes (NEA10, NEA11 and NEA12) isolated from perennial ryegrass and belonging to distinct taxa (E. festucae var. lolii, LpTG-2 and LpTG-3, respectively), and two endophytes isolated from tall fescue (belonging to the taxon E. coenophiala). Consistent with previous studies, endophyte viability following AA treatment was inversely correlated with both increasing RH and duration of treatment in both species. Significant interaction effects between cultivar variety, endophyte genotype, humidity level and treatment duration were observed for both seed germination and endophyte viability. NEA12 consistently displayed higher viability following AA treatment than NEA10 and NEA11 in each of the perennial ryegrass cultivars. Stable host-endophyte combinations were observed for 'Alto'-NEA10, 'Bealey'-NEA11 and 'Trojan'-NEA12, 'Bronsyn' being the least compatible host population. The applicability of AA for selecting stable host-endophyte associations, or to identify and eliminate unstable associations, is discussed.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: December 1, 2016

More about this publication?
  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Membership Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more