A convenient and accurate seed germination assay for root parasitic plants
Seed germination of the root parasitic plants Striga spp. and Orobanche spp. requires two steps: conditioning in one Petri dish and germinating in another dish. Expensive Whatman glass fibre filter paper is used in most germination studies of these plants. Therefore, this method is time consuming and costly. In this study, seed conditioning and germination were performed in the same Petri dish using an inverted filter paper method. This method provides a 'platform' for the seeds and therefore does not involve the use of Whatman glass fibre filter paper, thereby reducing costs, decreasing the length of time required and improving efficiency. This method can also be used for studies concerning the germination of herbs, vegetables and economic crops with minute seeds.
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Document Type: Research Article
Publication date: April 1, 2016
This article was made available online on February 16, 2016 as a Fast Track article with title: "A convenient and accurate seed germination assay for root parasitic plants".
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