Germination and cryopreservation responses of Jatropha curcas in relation to seed quality
Ex vitro and in vitro germination in relation to seed quality, cryopreservation and seedling regeneration were examined following liquid nitrogen storage of Jatropha curcas seeds and zygotic embryonic axes. Six seed weight categories were used: (1) ≤0.399 g; (2) 0.400 - 0.499 g; (3) 0.500 - 0.599 g; (4) 0.600 - 0.699 g; (5) 0.700 - 0.799 g; (6) ≥0.800 g. Germination was related to the weight and quality of seeds, the presence / absence of the seed coat and type of explant. The moisture content and water uptake during five hours imbibition were related to the initial fresh weight of seeds. The highest germination (90%) was similar under ex vitro and in vitro conditions for seeds with seed coat in the weight category 0.600 - 0.699 g. Shoot regeneration following liquid nitrogen (-196°C) storage was influenced by the type of explant used. Zygotic embryonic axes excised from cryopreserved seeds displayed the highest regeneration percentages with 95% compared with 64% obtained for isolated embryonic axes cryopreserved in plant vitrification solution droplets. Plants developed from cryopreserved seeds and isolated zygotic embryo axes were morphologically similar to the non-cryopreserved controls.
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Document Type: Research Article
Publication date: December 1, 2014
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