Dry seeds of Lactuca sativa (iceberg lettuce) and L. sativa cv. Hance salad that had imbibed water for 40 hours at 4°C (to moisture contents of 68 ± 3.9 and 62 ± 2.9%, respectively) followed by imbibition in 15 or 35% dimethyl sulfoxide (DMSO) for eight
hours, were cooled to various sub-zero temperatures (>-40°C) at 3 or 60°C hour-1 in a programmable freezer. After cooling to the various low temperatures, seeds were either thawed directly in a water bath at 40°C or plunged into liquid nitrogen for one week before
thawing at 40°C. Viability was assessed as the number of seeds that germinated at 21°C. The ice formation temperature, which was detected using a thermocouple during cooling (in the range of -12 to -40°C), did not affect subsequent germination following liquid nitrogen storage.
The higher concentration of DMSO was toxic whilst the lower concentration was essential for higher seed survival after both sub-zero and liquid nitrogen storage. Survival of both cultivars was best achieved after pre-cooling to -25 to -40°C. Pre-cooling at 3°C hour-1 was
effective for iceberg lettuce seeds but not for those of L. sativa Hance salad, implying a cultivar-specific effect. These findings provide important insights into the interaction of water content, pre-cooling, cooling rate and cryoprotectant concentration on seed storage.
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Document Type: Research Article
Institute of Biothermal Technology, University of Shanghai for Science and Technology, Shanghai 200093;, Email: [email protected]
Institute of Biothermal Technology, University of Shanghai for Science and Technology, Shanghai 200093
August 1, 2014
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