Seed coating with riboflavin, a natural fluorescent compound, for authentication of cucumber seeds
Seed authentication is a means to mark seeds to validate a particular seed lot and is an important aspect of seed quality assurance. The objective of this study was to develop an authentication test using a riboflavin-containing seed coating. The seed coating formulation was mixed with different concentrations of naturally fluorescent riboflavin in aqueous solutions to produce films. The viscosity and pH of these formulations were measured. Cucumber seeds were non-coated (treatment control) or coated with riboflavin at 0 (formulation control), 2, 3, 4 and 5% of the coating formulation. The addition of riboflavin to the seed coating formulations had little effect on film properties. Coated seed were packaged in re-sealable plastic bags and aged under ambient conditions for 10 months. For non-aged samples, germination of coated seeds did not differ from the non-coated seeds. Ageing decreased the percentage germination and germination rate for coated and non-coated seeds, but the coated seeds had the same or better germination than the non-coated control. The emission spectra for riboflavin-coated seeds were similar to that for pure riboflavin. Seed ageing did not diminish fluorescence. In summary, coating with riboflavin is an effective and convenient method to mark any cucumber seed lot for authenticity validation.
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Document Type: Research Article
Publication date: August 1, 2014
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