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Improvement of the tetrazolium test for evaluating tobacco (Nicotiana tabacum) seed viability

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The tetrazolium (TZ) test for evaluating tobacco seed viability is widely used for official and non-official applications. It is very difficult and time-consuming to do the TZ test for tobacco seeds since the seeds are extremely small. To overcome those disadvantages, the TZ test was improved in our research by methods of (a) omitting the preconditioning; (b) cutting seeds in half in batches instead of one-by-one by fixing a batch of seeds on the surface of a block of wheat flour dough; (c) using iodonitrotetrazolium chloride (INT) instead of the commonly used triphenyl tetrazolium chloride (TTC). Experiments were carried out to test the improved methods with two tobacco varieties. The results showed that the labour required was greatly reduced using our improved methods, the time of testing was shortened from 68-70 to 5.2 hours under the premise of similar cost and test results in this research.
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Document Type: Research Article

Publication date: April 1, 2014

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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