Skip to main content
padlock icon - secure page this page is secure

Improvement of the tetrazolium test for evaluating tobacco (Nicotiana tabacum) seed viability

Buy Article:

$35.00 + tax (Refund Policy)

The tetrazolium (TZ) test for evaluating tobacco seed viability is widely used for official and non-official applications. It is very difficult and time-consuming to do the TZ test for tobacco seeds since the seeds are extremely small. To overcome those disadvantages, the TZ test was improved in our research by methods of (a) omitting the preconditioning; (b) cutting seeds in half in batches instead of one-by-one by fixing a batch of seeds on the surface of a block of wheat flour dough; (c) using iodonitrotetrazolium chloride (INT) instead of the commonly used triphenyl tetrazolium chloride (TTC). Experiments were carried out to test the improved methods with two tobacco varieties. The results showed that the labour required was greatly reduced using our improved methods, the time of testing was shortened from 68-70 to 5.2 hours under the premise of similar cost and test results in this research.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: April 1, 2014

More about this publication?
  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Membership Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more