Screening fluorescent dyes for anti-counterfeit labelling in rape seed (Brassica napus)
Five fluorescent dyes – rhodamine B (RB), rhodamine 6G (R6G), lissamine rhodamine B (LRB), 3, 6-diamincacridine hulmisulfate (DH) and safranine T (ST) – were used to label seeds as an anti-counterfeiting technology. The effects of the d yes on physiological parameters of rape and the patterns of fluorescence of rape seedlings were investigated. The results showed that s oaking seeds for 24 hours in 0.1 mg RB ml−1, 0.1 mg LRB ml−1 or 0.1 mg ST ml−1 had no significant effects on seed germination, seedling growth, seedling protective enzyme activities or chlorophyll content. RB and LRB emitted bright red and bright orange fluorescence under a fluorescence microscope, when excited by green light (546 nm) and blue light (495 nm), respectively, whereas ST showed little difference compared with the control. Moreover, the vascular bundles of stem, seedling roots and aerial parts of seedlings treated with RB all emitted brilliant fluorescence for a long time, which could be used as a marker in seedlings. Our results suggest that labelling rape seeds with RB dye at a suitable concentration can be used as an anti-counterfeiting technique.
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Document Type: Research Article
Publication date: October 1, 2012
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