Genetic purity of common bean seed generations (Phaseolus vulgaris cv. 'INTA ROJO') as tested with microsatellite markers
The common bean cultivar 'INTA ROJO' possesses many superior traits, but the occurrence of off-type seeds and plants during seed production has hampered the certification process and questioned its genetic purity. To date, its genetic purity has been assessed using only phenotypic methods. However, this study used microsatellite markers to compare the genetic composition of different seed generations and to confirm the identity of off-type plants and seeds. Genotyping was conducted for twelve microsatellite loci, and comparisons of genotype frequencies were conducted using the breeder's seed as a reference population. Additionally, off-type seeds and plants, among them the off-type seeds known as “frijol viterra” and “frijol rojo oscuro”, were contrasted with 'INTA ROJO' seed through pairwise comparisons of genetic distances. The results suggest that changes in genotype frequencies take place during seed production and are ascribed to selection pressures caused by the environment and to inadequate roguing procedures. The off-type “frijol rojo oscuro” was probably derived from mutations and/or outcrossing among cultivars. In contrast, “frijol viterra”, although phenotypically distinct, was confirmed to belong to 'INTA ROJO'. Microsatellite markers were hence found to be useful for assessing the genetic purity of common bean seed generations and can complement the current phenotypic methods.
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Document Type: Research Article
Publication date: April 1, 2012
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