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Comparison of modified blotter and agar incubation methods for detecting fungi in Zinnia elegans seeds

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Seed health is one of the most important factors affecting seed quality. However, there is no standard method for detecting fungi in Zinnia elegans Jacq. seeds. Seven incubation methods for evaluation of zinnia seed health were compared: deep freeze blotter test, blotter test with polyethylene glycol, blotter test with mannitol, agar test on potato-dextrose-agar (PDA) after seed disinfection, agar test on PDA without seed disinfection, agar test on PDA with an addition of malachite green and agar test on PDA with an addition of rose bengal. Thirty fungal species or genera were associated with the seeds. Alternaria alternata, A. zinniae, Cladosporium spp. and Fusarium spp. were identified most frequently. The greatest infestation with most fungi, especially Alternaria spp. was observed in seeds tested by the deep freeze blotter test followed by the blotter test with mannitol and both of them could be recommended for further study on Z. elegans seeds health testing.
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Document Type: Research Article

Publication date: April 1, 2012

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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