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Germination and dormancy-breaking of Diren (Melastoma dodecandrum) seeds

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Melastoma dodecandrum (Melastomaceae) is a tropical and subtropical sub-shrub that grows in South China and North Vietnam. In this project, we studied the effect of various chemical pretreatments and short-term storage on its seed germination. Seeds were germinated at temperatures from 10 to 35°C, and 25 and 30°C were found to be the optimum temperatures for germination. Light provided by cool white fluorescent lamps at 800-1000 lux for 12h every day significantly improved seed germination. Soaking for 24 hours in 250-1000 mg/L gibberellic acid (GA3), 50-200 mg/L 6-Benzylaminopurine (6-BA) or 10%-30% potassium nitrate (KNO3) all significantly increased GP (germination percentage) and GI (germination index), while 10% hydrogen peroxide (H2O2) did not significantly improve GI, but increased the GP. Indole-3-butyric acid (IBA) at 50-200 mg/L and 20%-30% H2O2 solution did not significantly affect GP or GI. Dormancy was also relieved by after-ripening. Storage at 5, 10 and 25°C for 30-120 days all accelerated germination compared to the control, and GP and GI both increased more rapidly at 10 and 25°C than at 5°C as storage durations increased from 30 to 90 days. However at -10°C, GP and GI decreased with increased duration when stored for 30-120 days. To propagate seedlings of this sub-shrub, the seeds should be stored for 90-120 days at 15-25°C to ensure the completion of after-ripening. In addition appropriate application of chemical pretreatments can enhance germination.
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Document Type: Research Article

Publication date: April 1, 2012

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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