Skip to main content
padlock icon - secure page this page is secure

Fungal endophyte detection in pasture grass seed utilising the infection layer and comparison to other detection techniques

Buy Article:

$35.00 + tax (Refund Policy)

A new detection technique, which exploited the biology surrounding the colonisation of pasture grass embryos by the endophytic fungus Neotyphodium, was developed to assess the proportion of endophyte infected seeds within selected accessions. A generalized linear mixed model followed by one-sided pairwise comparisons were conducted to compare the new infection layer (IL) technique and a common seed squash (SS) to the tissue print-immunoblot (TPIB). The latter is a practice used within the seed industry to test endophyte viability within accessions of Lolium arundinaceum and Lolium perenne. The IL was found to be a simple and accurate technique for predicting the proportion of viable endophyte in uncompromised seed accessions and was more accurate than the SS, which frequently recorded an over estimation. However, neither the SS nor the IL were a substitute for TPIB in detecting viable endophyte in compromised seed accessions such as those affected by certain systemic fungicides.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: October 1, 2011

More about this publication?
  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Membership Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more