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An improved real-time PCR system for broad-spectrum detection of Didymella bryoniae, the causal agent of gummy stem blight of cucurbits

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Gummy stem blight is a major disease of cucurbit crops caused by Didymella bryoniae (anamorph Phoma cucurbitacearum). Previously, we used random amplified polymorphic DNA (RAPD) profiles and specific PCR primers to sort a worldwide collection of isolates of D. bryoniae into two major genotypes, RAPD Group (RG) I and RG II. A recently developed real-time PCR assay for D. bryoniae specific only for isolates with the RG I genotype has limitations in practical disease diagnosis. The objective of the present study was to develop a new set of primers and TaqMan probe in conjunction with real-time PCR to detect both genotypes. This new Didymella detection system generated strong Ct values to a worldwide collection of D. bryoniae including 105 RG I, 13 RG II and 7 unassigned isolates. This broad spectrum and specific real-time PCR system could be used in routine seed and plant health diagnosis for D. bryoniae.
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Document Type: Research Article

Publication date: October 1, 2010

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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