Standardization of the tetrazolium test in Baliospermum montanum (Willd.) Muell.-Arg.
The present investigation deals with the standardization of evaluation criteria of the tetrazolium test on the seeds of Baliospermum montanum (Willd.) Muell.-Arg. Firstly, standardization of optimum conditions for TZ staining was done. Imbibed seeds were subjected to different incubation temperatures, soaking time and concentration of tetrazolium solution. Clear staining pattern and good colour intensity were obtained when pre-moistened cut seeds were soaked in 0.1% TZ solution at 30°C for 17 hrs and 0.25% TZ solution at 20°C for 17 hrs. The standardization of the stain topography of viable and non-viable seeds was carried out and four staining patterns were recognized. Completely and uniformly stained seeds and seeds with the radicle tip stained and some parts of the cotyledons stained were considered as viable. The results of the TZ test were similar to the laboratory test carried on different viability seed lots generated by accelerated ageing. Among the above two treatments 0.1% solution at 30°C for 17 hrs is more suitable since the quantity of TZ salt used is less.
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Document Type: Research Article
Publication date: July 1, 2010
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