Seed priming and salinity induced variations in wheat (Triticum aestivum L.) leaf protein profile
Salinity and priming induced changes in electrophoretic profiles of leaf proteins were analyzed using SDSPAGE. Wheat (Auqab-2000) seeds were grown under saline (150 mM NaCl) and non-saline (H2O) conditions after five priming treatments i.e. hydropriming, freezing (-20°C), 100 mM CaCl2.2H2O, 0.28 mM ascorbate (AsA) and 0.36 mM salicylic acid (SA). Salinity caused the induction of a new protein with molecular weight of 38 kDa which was not observed in the control. This protein (38 kDa) was not repressed by priming treatments. Salinity also induced a 29 kDa protein which was completely repressed by priming treatments. Two peptides (27 and 25 kDa) disappeared under salinity. However, these peptides did not disappear on AsA, SA and CaCl2.2H2O priming. Most prominent priming induced protein (PIP) was a 36.7 kDa peptide which was expressed by all priming treatments under non-saline condition. The intensity of this peptide was higher in case of freezing and hydropriming. Priming with AsA, CaCl2.2H2O and SA induced a 36 kDa protein under non-saline condition. A 33.2 kDa protein was expressed by AsA and CaCl2.2H2O treatments under non-saline condition. Salinity and priming induced proteins may be antioxidants (SODs) and dehydrins.
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Document Type: Research Article
Publication date: April 1, 2010
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