Effect of PEG priming on plasma membrane H+-ATPase activities and mitochondrium function in soybean seeds
The soybean (Glycine max) Zhonghuang No.22 is sensitive to imbibitional chilling injury, which limits the productivity of agricultural crops severely. PEG priming can improve chilling tolerance of soybean seeds. On the base of primary results, the changes of plasma membranes
H+-ATPase activities, cotyledon mitochondrium respiration and oxidative phosphorylation activity responses in soybean seeds were investigated during PEG priming. The data showed that PEG priming resulted in a increase of the H+-ATPase hydrolytic activity in 12 h, then
the activity decreased gradually, but was higher than control. The H+-pumping activity increased quickly during PEG priming 24 h, then increased slowly by the end of 72 h. The results indicated that the state III respiration rates of mitochondria from primed seed were lower than
that of ADP stimulated control mitochondria when L-Mal, α-Kg and NADH were used as substrates respectively. On the contrary, the priming could significantly improve the oxidative phosphorylation activity of cotyledon mitochondria in 12 h, and the ADP/O value was consistent with those
of theoretical expectation. Thought when NADH was used as substrate, control seed mitochondria possessed oxidative phosphorylation activity, while ADP/O value was obvious lower than those of mitochondria from the primed. When Succ was used as substrate, the oxidative phosphorylation activity
of the primed seed had recovered normally in primed 24 h. When a different substrate was used, the emerging sequence of the oxidative phosphorylation activity of the primed seeds was as follows: 1. NADH (nicotinamide adenine dinucleotide), 2. α-Kg (α-ketoglutaric acid), 3. Succ
(succinic acid), 4. L-Mal (L-malic acid).
Document Type: Research Article
Publication date: 01 April 2010
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