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Assessment of viability of Jatropha curcas L. seeds using the tetrazolium test

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Assessment of the potential viability of Jatropha curcas L. was done using the Triphenyl Tetrazolium Chloride (TZ) test, and the results correlated with actual viability evaluated by laboratory germination. Seeds were subjected to different incubation temperatures, soaking time and concentration of tetrazolium solution. Soaking of the pre-moistened seeds in 0.1%TZ solution at 30°C for six hours or 0.25%TZ at 40°C for four hours produced clear staining pattern and good colour intensity and may be appropriate conditions for TZ testing in Jatropha. Seeds which are either completely and uniformly stained or were uniformly stained except for minor unstained areas on the cotyledons were considered viable. For these seeds there was a strong correlation with the laboratory germination of five seed lots.
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Document Type: Research Article

Publication date: July 1, 2009

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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