Impact of glyphosate tolerance gene on seed quality of transgenic bentgrass
There is a hypothetical risk that the glyphosate tolerance gene (CP4 EPSPS) may increase seed viability and vigour in transgenic glyphosate tolerant creeping bentgrass (GTCB) (Agrostis stolonifera L.), consequently increasing its persistence. This study was conducted to examine the impact of the CP4 EPSPS gene on seed viability, vigour, longevity, and germination energy of GTCB. Two GTCB events, ASR365 and ASR368, were tested under four germination environments: (1) standard germination test (SGT = 15/25°C for 28d), (2) Suboptimal temperature germination test (SubGT = 14°C for 28d), (3) Supra-optimal temperature germination test (SupraGT = 32°C for 28d) and (4) accelerated aging test (AAT = 45°C for 30 h). Potential gene effects were evaluated by comparing the stress effects of low and high temperatures induced by the SubGT, SupraGT and AAT environments on resistant (RR) and susceptible (RS) progeny within each transgenic event; and by comparing the percentage of RR progeny from the SGT to the percentage of RR progeny from stress environments (i.e., SubGT, SupraGT and AAT). Conventional cultivars of creeping bentgrass, 'SR1020', 'Backspin', and a dryland bentgrass, 'Highland' (A. castellana) were included for comparative purposes. The study was repeated twice. Under all germination environments used in the study, the glyphosate tolerance gene did not provide superior viability, vigour, or longevity to the GTCB. In a few cases, the high vigour plants within the resistant progeny showed better germination than the low vigour plants, but this was attributed to seed vigour effects rather than gene effect.
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Document Type: Research Article
Publication date: July 1, 2009
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