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GA3 enhances in vitro seed germination in Santalum album

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Emergence and cultivation of Santalum album, an important tree species is reported to be poor and unpredictable as a result of low and delayed germination. The present paper describes a quick and reliable method that ensures fast and uniform seed germination of S. album. The endocarp was removed under aseptic conditions and the seeds pre-treated with 2, 4, 6 and 8 mM gibberellic acid (GA3) for 12 h, then inoculated on Murashige and Skoog's (MS) medium fortified with or without 2.0 or 4.0 μM benzylamino purine (BA). The imbibition of seeds in 4 mM GA3 not only improved final germination to 80.67% in 30 days as compared to 46% germination in non-treated seeds, but also reduced mean germination time. The presence of 4.0 μM BA in the MS medium improved the germination even further. Polyembryony was observed in about 2 to 3% seeds. Even though the S. album is considered as a hemi-root parasite, the resultant saplings were maintained for about two years in the absence of host. The technique can effectively be used for raising large numbers of saplings in a relatively short time.
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Document Type: Research Article

Publication date: July 1, 2009

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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