Breaking seed dormancy in long-term stored seeds from Iranian wild almond species
Different treatments for germination of two-year stored seeds have been assayed for some wild almond species including Prunus scoparia, Prunus elaeagnifolia and Prunus hausskenchtii. The treatments included the application under in vivo stratification (chill) conditions of distilled water, hydrogen peroxide, and GA3; and the applications of different concentrations of GA3 (0.5, 0.75 and 1 mg/l) in agar medium under in vitro conditions. Seed germination commenced after 5 and 10 days under in vivo and in vitro conditions, respectively. Significant differences were observed in the final percentage of seed germination between species and treatments in both experiments and which collectively showed that P. scoparia had the best germination capacity. Treatments with H2O2 and GA3 were significantly better in all populations with a higher percentage of germination in the in vivo stratification experiments. In addition, seed germination was affected by hormone concentration and revealed a clear difference with increasing GA3 concentration in vitro. A combination of stratification and a pre-treatment with H2O2 and GA3 reduced the germination time and promoted a higher germination rate of the stored seeds in the wild almond species studied.
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Document Type: Research Article
Publication date: July 1, 2009
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