Skip to main content
padlock icon - secure page this page is secure

Molecular testing of cucumber hybrid genetic purity with RAPD marker

Buy Article:

$35.00 + tax (Refund Policy)

The molecular marker of Random amplified polymorphic DNA (RAPD) was used to evaluate seed genetic purity of a novel commercial hybrid cultivar 'Biyu' in cucumber. Out of 395 primers, 44 were found to be fit for the genetic purity identification of cucumber hybrid seeds. The results showed that 15 primers including OPBH-04 and OPF-20 could produce female parent-specific (FPS) markers, and 15 primers including OPBC-20 and OPO-07 could produce male parent-specific (MPS) markers. With four primers of these two types, the genotypes of 220 cucumber hybrid individuals were identified. It was found that two false hybrid individuals that may come from mixture of other cultivars, and one individual which was derived from selfing male parent plant, were identified. Consequently, the genetic purity of the cucumber cultivar 'Biyu' hybrid seed lot was 98.64%. The result from molecular testing was in well accordance with that from purity inspecting in the field plot. Therefore, the molecular marker of RAPD is a high efficient and accurate technique for genetic purity testing of commercial hybrid seeds in cucumber.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: July 1, 2008

More about this publication?
  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Membership Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more