Comparison of characteristics of SRAP and SSR markers in genetic diversity analysis of cultivars in Allium fistulosum L.
Both SRAP and SSR were used to analyze genetic diversity of 20 cultivars in Allium fistulosum L. 256 SRAP primer pairs and 17 SSR primers were tested. In SRAP analysis, 161 (62.9%) primer pairs generated a total of 336 polymorphic bands. The number of polymorphic bands detected by an individual primer pair ranged from 1 to 6, with an average number of 2.1. Genetic similarity (GS) coefficients among 20 cultivars based on the SRAP data ranged from 0.464 to 0.938, with a mean GS coefficient of 0.703. In SSR analysis, 13 (76.5%) primers generated a total of 52 polymorphic bands. The number of polymorphic bands detected by an individual primer ranged from 1 to 10, with an average number of 4.00. Genetic similarity (GS) coefficients among 20 cultivars based on the SSR data ranged from 0.404 to 0.885, with a mean GS coefficient of 0.712. Polymorphic SRAPs and SSRs were abundant in genetic diversity analysis among closely related cultivars. However, with the morphological traits as a standard, the information given by SRAP was more accordant to reflect the morphological variability than that of SSR marker. On the whole, SRAP was suggested to be a preferred choice to analyze genetic diversity, and SSR could be chosen firstly in genetic purity test and identification of cultivars in Allium fistulosum L.
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Document Type: Research Article
Publication date: July 1, 2008
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