Skip to main content
padlock icon - secure page this page is secure

Effect of cryopreservation, gibberellic acid and mechanical scarification on the seed germination of eight endemic species from the Canary Islands

Buy Article:

$35.00 + tax (Refund Policy)

Seed germination of eight endemic species of the Canary Islands (Descurainia burgaeana, Lavatera acerifolia, Lotus mascaensis, Pimpinella cumbrae, Ranunculus cortusifolius, Scilla haemorrhoidalis, Solanum vespertilio and Spartocytisus supranubius) was studied after seed storage in liquid nitrogen (LN, −196 °C), soaking in gibberellic acid (GA3) and mechanical scarification. Lotus mascaensis and Pimpinella cumbrae are classified as "vulnerable" (VU) and Solanum vespertilio as "critically endangered" (CR) according to the IUCN (International Union for the Conservation of Nature) criteria. The germination trials were carried out under controlled conditions of light and temperature. For six of the eight species studied, the final germination percentages of cryopreserved seeds were higher than those of control seeds (non-cryopreserved seeds). Furthermore for three of the species this increase was significant. On the other hand for two of the species, seeds that had been cryopreserved reached a germination percentage lower (Solanum vespertilio) and equal (Spartocytisus supranubius) to the control seeds, however these decreases were not significant. The germination of Solanum vespertilio was significantly enhanced by soaking of the seeds in gibberellic acid (GA3) and the scarified seeds of Lotus mascaensis and Spartocytisus supranubius reached a significantly higher germination percentage than untreated seeds. The germination rate, expressed by the mean germination time (MGT), was significantly lower for two samples of cryopreserved seeds, three samples of seeds pretreated with GA3 and three samples of scarified seeds. Therefore, this study proves seed cryopreservation is a suitable procedure for the long-term seeds conservation of several endemic species from the Canary Islands.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: April 1, 2008

More about this publication?
  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Membership Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more