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Effect of scarification on seed germination of red clover in hydrotime model terms

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The objectives of this study were to determine water relationships during germination of red clover seeds treated with concentrated sulfuric acid and to test whether breaking physical dormancy (hard seed) is associated with changes in parameters of the hydrotime model. Intact and scarified seeds of cultivars Krynia and Bona were incubated at reduced water potentials determined by utilizing polyethylene glycol solutions. Seed germination time courses were analyzed by probit regression according to the population-based hydrotime model. The high values of R2, applied as a measure of goodness of fit, showed that the hydrotime model worked well to characterize germination time courses of red clover seeds at reduced water potentials. The coefficient of variation values obtained for mean base water potential (ψ b (50)) and its standard deviation (σψb ) during scarification period was lower than the values obtained for hydrotime constant ( H ). This suggested that braking physical dormancy in Trifolium pratense seeds of both cultivars would be characterized by a significant decrease of  H value, while the other parameters of the model are relatively constant. The application of the hydrotime model to describe and predict breaking of physical dormancy may be an effective approach, especially for species of arable plants, such as red clover, which do not contain a great number of hard seeds.
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Document Type: Research Article

Publication date: July 1, 2007

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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