Skip to main content
padlock icon - secure page this page is secure

Pressure – time dependency of vacuum degassing as a rapid method for viability assessment using tetrazolium chloride: a comparative study of 17 Pinus species

Buy Article:

$35.00 + tax (Refund Policy)

Vacuum degassing of seeds in tetrazolium chloride (TZ) solution has been proposed as an alternative and rapid method for assessing pine seed viability. In this study, we determined the effects of both suction level and the subsequent period of stain development at 30°C, on the viability estimates for Pinus caribaea and P. pinea. Our results demonstrated that three, 10 min cycles of degassing increased viability estimates, with the highest suction level we applied (96 kPa) being the most effective. In addition, at 96 kPa of suction the subsequent period of stain development could be reduced to 3 h whilst still retaining an equivalent estimate of viability to both a standard ISTA TZ test (18 h at 30°C) and germination level. Subsequently, for a further 17 Pinus seed lots, the estimate of viability from three cycles of degassing at 96 kPa followed by 3 h of stain development, was compared with both an 18 h ISTA test and germination. These results indicated that compared to germination levels, degassing provided an equivalent or improved estimate of viability to the ISTA method. Consequently, the application of degassing at 96 kPa, followed by 3 h of stain development at 30°C should allow rapid and reliable viability assessment for a wide range of Pinus species.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: July 1, 2006

More about this publication?
  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Membership Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more