Real Time Polymerase Chain Reaction (PCR) quantification of T25 maize seeds – influence of the genetic structures in the maize kernel on the quantitative analysis
Real Time PCR is a technique widely used for GMO quantification. The maize seed contains diploid embryo and triploid endosperm. This implies that the ratio transgene/endogene determined by Real Time PCR will vary due to the presence of different genome copies in the seed compartments. The transgene/endogene ratio is determined for single seeds from commercial transgenic line T25. This ratio is compared between the seeds that originate from reciprocal crosses between T25 and the conventional variety B73. The variation in the GMO content due to the presence of seeds with different origin in the seed samples of 3000 seeds is evaluated.
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Document Type: Research Article
Publication date: July 1, 2006
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