Rapid characterisation of some species of the genus Onobrychis Miller (Leguminosae: Papilionoideae) by isozyme methods
Nine taxa of the genus Onobrychis (O. viciifolia, a crop, and eight wild relatives) have been compared though electrophoretic profiles of ten isozyme systems. The band patterns obtained using bulking seeds, identified samples faster than other methodologies, which could be useful in seed banks management. The enzyme profiles obtained provided 114 reliable bands, which were scored as multistate traits (not with allelic value). In order to use this method for an approach to taxonomic purposes, the relationships among taxa were estimated by dendrograms based on the similarity matrix treated by the unweighted pair group method with arithmetical averages (UPGMA). The results are in agreement with the recognised taxonomy, and the nine Onobrychis species were clustered in a main group that includes the species of subsection Vulgatae, and the taxa belonging to subsections Hispanicae, Brachysemiae and Macropterae clearly appear differentiated.
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Document Type: Research Article
Publication date: July 1, 2006
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