Artificial ageing of Brassica seeds of different maturity levels
Brassica crops have an extended flowering period. Therefore, the seeds harvested at a particular time comprise a lot of mixed maturity. The seeds from proximal and distal positions on inflorescence of Brassica campestris (cvs TL15 and PBT37) were collected separately and subjected to accelerated ageing. Accelerated ageing for 96 h led to a marked decline in rate of germination and seedling vigour as compared to control, i.e., unaged seeds, but those collected from proximal position of inflorescence had higher rate of germination and seedling vigour than seeds collected from distal position. The membrane permeability in terms of electrical conductivity of seed increased significantly in seeds aged for 96h as compared with unaged seeds. Hydropriming of aged seeds reduced the electrolyte leakage compared with aged unprimed seeds because of repair of seed membrane during hydration. Accelerated ageing led to a significant increase in the level of starch and total sugars while protein content decreased. Ageing also affected banding pattern of mustard seed globulins when subjected in PAGE and this effect was more pronounced in seeds collected from distal rather than proximal position. Hydropriming restored the activities of invertase and amylases with a consequent decrease in the amount of sugars and total starch.
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Document Type: Research Article
Publication date: July 1, 2006
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