Investigation into the feasibility of assessing the hybridity of immature rice grains
To investigate the possibility of testing hybrid purity of two-line hybrid rice before harvest, ultrathin-layer isoelectric focusing (UTLIEF) of seed proteins was used to study the accumulation patterns of male marker bands (MMBs) in developing F1 seeds. It was found that in F1 seeds of early-rice of all five combinations (Peiai 64S/Peifu, Peiai 64S/G67, Peiai 64S/Minkezhan, Peiai 64S/I9-1 and Peiai 64S/EP431) tested in this research, MMBs began to accumulate at the developmental stage of 7 days after pollination (DAP) and presented clearly and stably in all F1 seeds from 11 DAP up. However, in F1 seeds of late-rice of five combinations, time for the first appearance of MMBs and for all F1 seeds to contain clear and stable MMBs varied from 9 to 15 DAP and 13 to 20 DAP, respectively. It was concluded that hybrid purity testing of two-line hybrid rice could be carried out before harvest.
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Document Type: Research Article
Publication date: April 1, 2006
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