Skip to main content
padlock icon - secure page this page is secure

Variability in response of seeds to liquid nitrogen exposure in wild coffee (Coffea arabica L.)

Buy Article:

$35.00 + tax (Refund Policy)

In order to investigate the intraspecific variability for tolerance to liquid nitrogen (LN) exposure of coffee (Coffea arabica) seeds, seed and embryo viability after desiccation or desiccation and cooling to −196°C, with or without controlled rehydration of seeds after thawing, has been studied within a core subset of 30 accessions of the CATIE field genebank. A high variability for seed sensitivity to LN exposure was observed: seed viability after cryopreservation varied from 0 to 74% among the accessions studied (0 to 100% when expressed in percentage of the desiccation control) and was not correlated to seed size, seed water content after desiccation, or seed and embryo viability after desiccation. By contrast, no variability was found between the genotypes studied for embryo viability after LN exposure. Viability of embryos after cryopreservation was always very high, indicating that, when occurring, the decline or loss of seed viability after LN exposure was due to damages to the endosperm only. Controlled post-thaw rehydration of seeds, through a 6-week osmoconditioning treatment with a −1.25 MPa polyethylene glycol solution, dramatically increased viability of frozen seeds. However, this effect was variable within the accessions studied and independent of seed viability after desiccation or desiccation and LN exposure.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Publication date: July 1, 2005

More about this publication?
  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Membership Information
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more